Strain-specific contribution of NS1-activated phosphoinositide 3-kinase signaling to influenza a virus replication and virulence

Ayllon, J., Hale, B.G. and Garcia-Sastre, A. (2012) Strain-specific contribution of NS1-activated phosphoinositide 3-kinase signaling to influenza a virus replication and virulence. Journal of Virology, 86(9), pp. 5366-5370. (doi: 10.1128/JVI.06722-11)

Full text not currently available from Enlighten.

Abstract

We generated influenza A viruses expressing mutant NS1 proteins unable to activate phosphoinositide 3-kinase (PI3K) in two mouse-lethal strains. The recombinant A/Puerto Rico/8/34 (rPR8) mutant virus strain was attenuated and caused reduced morbidity/mortality. For the recombinant A/WSN/33 (rWSN) virus strain, the inability to stimulate PI3K had minimal impact on replication or morbidity/mortality. Cell-based assays revealed subtly distinct intracellular sites of NS1 localization and PI3K activation between the strains. We hypothesize that specific spatially regulated NS1-activated PI3K signaling, rather than simply the total level of active PI3K, is important for virus replication and virulence.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hale, Dr Benjamin
Authors: Ayllon, J., Hale, B.G., and Garcia-Sastre, A.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Journal of Virology
ISSN:0022-538X
ISSN (Online):1098-5514
Published Online:15 February 2012

University Staff: Request a correction | Enlighten Editors: Update this record