Multivalent carbohydrate recognition on a glycodendrimer‐functionalized flow‐through chip

Branderhorst, H.M., Ruijtenbeek, R., Liskamp, R.M.J. and Pieters, R.J. (2008) Multivalent carbohydrate recognition on a glycodendrimer‐functionalized flow‐through chip. ChemBioChem, 9(11), pp. 1836-1844. (doi:10.1002/cbic.200800195)

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Abstract

Dendrimers were fitted out with up to eight mannose moieties by “click” chemistry. They were subsequently attached to aluminum oxide chips via a spacer that was linked to the dendrimer core; this resulted in a microarray of glycodendrimers. Binding of the glycodendrimers to the fluorescent lectins ConA and GNA was observable in real time. In a single experiment it was possible to observe the multivalency enhancement or cluster effect in the binding event. This effect was small for ConA, in agreement with its widely spaced binding sites, whereas it was large for GNA, with its twelve much more closely spaced binding sites. The dendrimer-fitted chip represents a valuable screening tool for multivalency effects. Furthermore kinetic and thermodynamic data on binding events can be deduced. Inhibition experiments are also possible with the system as was shown for ConA with α-methyl mannose as the inhibitor.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Liskamp, Professor Robert
Authors: Branderhorst, H.M., Ruijtenbeek, R., Liskamp, R.M.J., and Pieters, R.J.
College/School:College of Science and Engineering > School of Chemistry
Journal Name:ChemBioChem
Publisher:Wiley
ISSN:1439-4227
ISSN (Online):1439-7633
Published Online:04 July 2008

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