Bovine intelectins: cDNA sequencing and expression in the bovine intestine

Blease, S.C., French, A.T., Knight, P.A., Gally, D.L. and Pemberton, A.D. (2009) Bovine intelectins: cDNA sequencing and expression in the bovine intestine. Research in Veterinary Science, 86(2), pp. 254-256. (doi:10.1016/j.rvsc.2008.06.002)

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Abstract

Intelectins (Itlns) are lectins with potential roles in innate immunity, capable of binding bacteria via galactofuranose residues. Itlns also function as intestinal receptors for the antimicrobial glycoprotein lactoferrin (Lf). Since Lf binds strongly to enterohemorrhagic Escherichia coli O157:H7 (EHEC), we aimed to determine the expression of Lf receptor in terminal rectum, the site of predilection of EHEC in cattle. We sequenced two bovine intelectins (Itln1 and Itln2) and showed that both were expressed in abomasum and rectum, but expression appeared minimal in the jejunum. There was significantly higher expression of Itln2 in terminal rather than proximal rectum. Lactoferrin was expressed in all samples examined. Thus, we have demonstrated two novel bovine Itlns and shown that they are expressed along with Lf in the gastrointestinal tract, where they may interact with microbial pathogens.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:French, Professor Anne
Authors: Blease, S.C., French, A.T., Knight, P.A., Gally, D.L., and Pemberton, A.D.
College/School:College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Research in Veterinary Science
ISSN:0034-5288

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