Human cord blood CD133+ cells exacerbate ischemic acute kidney injury in mice

Burger, D., Gutsol, A., Carter, A., Allan, D.S., Touyz, R.M. and Burns, K.D. (2012) Human cord blood CD133+ cells exacerbate ischemic acute kidney injury in mice. Nephrology Dialysis Transplantation, 27(10), pp. 3781-3789. (doi: 10.1093/ndt/gfs110)

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Abstract

<p>Background. Acute kidney injury (AKI) in humans has few therapeutic options. In experimental models, administration of progenitor cells facilitates recovery from AKI. Human umbilical cord-derived CD1331 progenitor cells promote endothelial repair in ischemic limb, heart and brain tissue.</p> <p>Methods. We examined the effects of human CD1331 progenitor cells in bilateral ischemia–reperfusion (I/R) kidney injury in non-obese diabetic severe combined immunode- ficient mice. CD1331 cells from human cord blood were injected intravenously at the time of reperfusion and the extent of injury was determined by plasma biochemistry and kidney histology.</p> <p>Results. In mice with I/R, fluorescently labeled CD1331 cells were detected in blood 2 min after injection but decreased rapidly thereafter with no evidence of homing to the kidneys. In mice subjected to I/R, CD1331 cells significantly increased plasma urea and Cr at 24 h compared to vehicle- or CD133- cell-treated mice. CD1331 cells exacerbated tubular necrosis and apoptosis, increased plasma tumor necrosis factor-a and increased kidney neutrophil infiltration. In contrast, CD1331 cells did not affect tubular cell proliferation. Administration of CD1331 cells to FVB/N mice post-I/R also augmented kidney injury.</p> <p>Conclusions. These data indicate that human cord bloodderived CD1331 cells unexpectedly exacerbate ischemic AKI in mice, possibly through soluble factors. Our study highlights the importance of caution in cell-based therapies for human AKI.</p>

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Touyz, Professor Rhian
Authors: Burger, D., Gutsol, A., Carter, A., Allan, D.S., Touyz, R.M., and Burns, K.D.
College/School:College of Arts > School of Culture and Creative Arts
Journal Name:Nephrology Dialysis Transplantation
ISSN:0931-0509
Published Online:04 May 2012

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