Label-free segmentation of co-cultured cells on a nanotopographical gradient

Reynolds, P.M. , Pedersen, R.H., Stormonth-Darling, J., Dalby, M.J. , Riehle, M.O. and Gadegaard, N. (2013) Label-free segmentation of co-cultured cells on a nanotopographical gradient. Nano Letters, 13(2), pp. 570-576. (doi: 10.1021/nl304097p)

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The function and fate of cells is influenced by many different factors, one of which is surface topography of the support culture substrate. Systematic studies of nanotopography and cell response have typically been limited to single cell types and a small set of topographical variations. Here, we show a radical expansion of experimental throughput using automated detection, measurement, and classification of co-cultured cells on a nanopillar array where feature height changes continuously from planar to 250 nm over 9 mm. Individual cells are identified and characterized by more than 200 descriptors, which are used to construct a set of rules for label-free segmentation into individual cell types. Using this approach we can achieve label-free segmentation with 84% confidence across large image data sets and suggest optimized surface parameters for nanostructuring of implant devices such as vascular stents.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Dalby, Professor Matthew and Riehle, Dr Mathis and Pedersen, Mr Rasmus and Reynolds, Dr Paul and Gadegaard, Professor Nikolaj
Authors: Reynolds, P.M., Pedersen, R.H., Stormonth-Darling, J., Dalby, M.J., Riehle, M.O., and Gadegaard, N.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Nano Letters
Publisher:American Chemical Society
ISSN (Online):1530-6992
Published Online:20 December 2012
Copyright Holders:Copyright © 2012 American Chemical Society
First Published:First published in Nano Letters 13(2):570-576
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
470561DTC in cell and proteomic technologies (continuation)Jonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/F500424/1ENG - BIOMEDICAL ENGINEERING