ETA and ETB receptors on vascular smooth muscle cells from mesenteric vessels of SHR

Schiffrin, E.L., Larivière, R. and Touyz, R.M. (1995) ETA and ETB receptors on vascular smooth muscle cells from mesenteric vessels of SHR. Clinical and Experimental Pharmacology and Physiology, 22(s1), S193-S194. (doi: 10.1111/j.1440-1681.1995.tb02877.x)

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1. To investigate the contribution of ET<sub>A</sub> and ET<sub>B</sub> receptors, calcium responses to the ET<sub>B</sub> agonist, IRL-1620, to endothelin-1 (ET-1) and to the ET<sub>A</sub> antagonist, BQ-123, were examined in primary cultured unpassaged vascular smooth muscle cells (VSMC) from mesenteric vessels of 3, 9 and 17 week old spontaneously hypertensive rats (SHR), Wistar and Wistar-Kyoto (WKY) rats using Fura-2 methodology. 2. IRL-1620 (10-7mol/L) and ET-1 (10-9mol/L) increased [Ca2+]i in all strains and ages. Responses to ET-1 and IRL-1620 were blunted in 17 week SHR. BQ-123 significantly reduced ET-1-stimulated [Ca2+]i. In endothelium-denuded mesenteric vessels, ET-1 and IRL-1620 induced significant [Ca2+]i responses. 3. Binding of ET-1 was significantly lower in mesenteric artery membranes from 17 week SHR compared to controls. 4. Thus, ET<sub>B</sub> receptors are present in rat mesenteric VSMC. In adult SHR, a reduced density of ET receptors results in decreased responses to IRL-1620 and to ET-1.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Touyz, Professor Rhian
Authors: Schiffrin, E.L., Larivière, R., and Touyz, R.M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Clinical and Experimental Pharmacology and Physiology
ISSN (Online):1440-1681
Published Online:28 June 2007

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