The effect of angiotensin II on platelet intracellular free magnesium and calcium ionic concentrations in essential hypertension

Touyz, R.M. and Schiffrin, E.L. (1993) The effect of angiotensin II on platelet intracellular free magnesium and calcium ionic concentrations in essential hypertension. Journal of Hypertension, 11(5), pp. 551-558.

Full text not currently available from Enlighten.

Publisher's URL: http://journals.lww.com/jhypertension/Abstract/1993/05000/The_effect_of_angiotensin_II_on_platelet.11.aspx

Abstract

Objective: To assess the effects of angiotensin II on intracellular free Mg2+ and Ca2+ concentrations in platelets from normotensive and hypertensive subjects.

Design and methods: Seventeen normotensive, 25 untreated hypertensive and 18 treated hypertensive patients were studied. Intracellular Mg2 + concentrations were measured with the fluorescent dye mag-fura-2-acetyoxymethylester (AM) and intracellular Ca2+ concentrations with the fluorescent dye fura-2AM under basal conditions and after stimulation by angiotensin II, saralasin (angiotensin II antagonist), arginine vasopressin and endothelin-1. The effects of increased extracellular Mg2 + concentrations on intracellular Mg2 + and Ca2+ concentrations were also determined.

Results: The intracellular basal Ca2+ concentration was significantly higher in the untreated hypertensives compared with the normotensives and treated hypertensive subjects (150+/-14nmol/l versus 120+/-17nmol/l for normotensives and 124+/-8nmol/l for treated hypertensives). The basal intracellular Mg2 + concentration was significantly lower in the untreated hypertensive compared to the normotensive and treated hypertensive groups (0.37 +/- 0.08 (imol/l versus 0.58 +/- 0.09 u.mol/1 for normotensives and 0.52 +/-0.11 u.mol/1 for treated hypertensives). In the hypertensive group, inverse correlations were found between intracellular Ca2+ and intracellular Mg2 + concentrations (r=-0.44, P < 0.05) and between intracellular Mg2 + and diastolic blood pressure (r=-0.35, P < 0.05), while a positive correlation was found between intracellular Ca2+ and systolic blood pressure (r=0.41, P < 0.05). Exposure of the platelets to 1 nmol/l angiotensin II significantly increased intracellular Ca2+ and significantly decreased intracellular Mg2 + concentrations in all three groups. The angiotensin ll-evoked effect on intracellular Ca2+ was exaggerated in the untreated hypertensives and blunted in the treated patients (basal versus stimulated: 150+/-14 versus 217+/-20nmol/l in untreated hypertensives; 124+/-8 versus 140+/-10nmol/l in treated hypertensives). Saralasin (0.1 u,mol/l) abolished the effects of angiotensin. Arginine vasopressin (1 u,mol/l) increased the intracellular Ca2+ concentration, whereas endothelin-1 (1 nmol/l) had no significant effect on either intracellular Ca2+ or intracellular Mg2 +. Increasing extracellular Mg2 + concentrations led to significant reductions in intracellular Ca2+ concentrations in all groups and a significant elevation of the intracellular Mg2 + concentration in the untreated hypertensive patients only.

Conclusions: These data demonstrate a relationship between angiotensin II and intracellular magnesium and calcium. In hypertension, angiotensin ll-stimulated calcium responses may be related to simultaneously decreased intracellular magnesium concentrations.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Touyz, Professor Rhian
Authors: Touyz, R.M., and Schiffrin, E.L.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Journal of Hypertension
Publisher:Lippincott Williams & Wilkins
ISSN:0263-6352

University Staff: Request a correction | Enlighten Editors: Update this record