A method for determining the total magnesium, calcium, sodium and potassium contents of human platelets

Touyz, R.M. and Milne, F.J. (1991) A method for determining the total magnesium, calcium, sodium and potassium contents of human platelets. Mineral and Electrolyte Metabolism, 17(3), pp. 173-178.

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Abstract

Platelet levels of magnesium, calcium, sodium and potassium may provide a better index of intracellular cations or total body ion status than serum, erythrocyte or leukocyte cation concentrations. A method is described for the determination of the total magnesium, calcium, sodium and potassium contents of human platelets. The method involves platelet isolation, repeated washing, lysis and measurement of the ions in the lysate. Magnesium and calcium were determined by atomic absorption spectroscopy and sodium and potassium by flame photometry. Platelet total potassium, magnesium, calcium and sodium were 4.5 +/- 1.5, 2.3 +/- 0.6, 1.8 +/- 0.5, 1.5 +/- 0.6 mumol/1 x 10(8) cells, respectively (means +/- SD). The method is simple, repeatable and accurate.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:UNSPECIFIED
Authors: Touyz, R.M., and Milne, F.J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Mineral and Electrolyte Metabolism
ISSN:0378-0392
ISSN (Online):1423-016X

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