Expression of tumor necrosis factor [alpha] converting enzyme in endocrine cancers

Kirkegaard, T., Naresh, A., Sabine, V.S., Tovey, S.M., Edwards, J., Dunne, B., Cooke, T.G., Jones, F.E. and Bartlett, J.M.S. (2008) Expression of tumor necrosis factor [alpha] converting enzyme in endocrine cancers. American Journal of Clinical Pathology, 129, pp. 735-743. (doi: 10.1309/N6YB6YDVF58YCNHN)

Abstract

Tumor necrosis factor [alpha] converting enzyme (TACE) mediates shedding of human epidermal growth factor receptor-4 (HER4). Recent data suggest that released HER4 intracellular domain (4ICD) induces apoptosis in breast cancer. TACE expression, as measured by immunohistochemical analysis, was observed in 183 of 383 breast carcinomas, 39 of 217 ovarian carcinomas, and 16 of 24 and 17 of 24 hormone-sensitive and hormone-insensitive prostate carcinomas, respectively. HER4 expression was detected in breast carcinomas by using 2 antibodies recognizing an extracellular or intracellular epitope. TACE expression was predominantly seen in tumors with high levels of 4ICD and membranous HER4. Apoptotic activity was measured by the terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) assay and cleaved caspase-3 staining in breast carcinomas. There was no significant association between cleaved caspase-3 or TUNEL positivity and 4ICD, whereas TUNEL positivity was seen predominantly in tumors with high levels of internalized HER4. The data presented herein show TACE expression in endocrine cancers and further support a role for TACE in breast cancer apoptosis.

• Item Type: Articles
• Additional Information: This is not the final published version
• Keywords: Tumor necrosis factor [alpha] converting enzyme, TACE, HER4, endocrine cancers, apoptosis, immunohistochemistry
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Edwards, Professor Joanne and Tovey, Ms Sian and Cooke, Prof Timothy
• Authors: Kirkegaard, T., Naresh, A., Sabine, V.S., Tovey, S.M., Edwards, J., Dunne, B., Cooke, T.G., Jones, F.E., and Bartlett, J.M.S.
• Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: American Journal of Clinical Pathology
• Publisher: Lippincott, Williams & Wilkins
• ISSN: 0002-9173
• ISSN (Online): 1943-7722
• Copyright Holders: Copyright © 2008 Lippincott, Williams & Wilkins
• First Published: First published in American Journal of Clinical Pathology 129:735-743
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher.