The 2-methylcitrate cycle is implicated in the detoxification of propionate inToxoplasma gondii

Limenitakis, J., Oppenheim, R.D., Creek, D.J., Foth, B.J., Barrett, M.P. and Soldati-Favre, D. (2013) The 2-methylcitrate cycle is implicated in the detoxification of propionate inToxoplasma gondii. Molecular Microbiology, 87(4), pp. 894-908. (doi:10.1111/mmi.12139)

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Abstract

Toxoplasma gondii belongs to the coccidian subgroup of the Apicomplexa phylum. The Coccidia are obligate intracellular pathogens that establish infection in their mammalian host via the enteric route. These parasites lack a mitochondrial pyruvate dehydrogenase complex but have preserved the degradation of branched-chain amino acids (BCAA) as a possible pathway to generate acetyl-CoA. Importantly, degradation of leucine, isoleucine and valine could lead to concomitant accumulation of propionyl-CoA, a toxic metabolite that inhibits cell growth. Like fungi and bacteria, the Coccidia possess the complete set of enzymes necessary to metabolize and detoxify propionate by oxidation to pyruvate via the 2-methylcitrate cycle (2-MCC). Phylogenetic analysis provides evidence that the 2-MCC was acquired via horizontal gene transfer. In T. gondii tachyzoites, this pathway is split between the cytosol and the mitochondrion. Although the rate-limiting enzyme 2-methylisocitrate lyase is dispensable for parasite survival, its substrates accumulate in parasites deficient in the enzyme and its absence confers increased sensitivity to propionic acid. BCAA is also dispensable in tachyzoites, leaving unresolved the source of mitochondrial acetyl-CoA.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Barrett, Professor Michael and Creek, Dr Darren
Authors: Limenitakis, J., Oppenheim, R.D., Creek, D.J., Foth, B.J., Barrett, M.P., and Soldati-Favre, D.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Molecular Microbiology
Publisher:Wiley-Blackwell
ISSN:0950-382X
ISSN (Online):1365-2958
Published Online:11 January 2013
Copyright Holders:Copyright © 2012 Blackwell Publishing Ltd
First Published:First published in Molecular Microbiology 87(4):894-908
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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