Truncation of TRIM5 in the Feliformia explains the absence of retroviral restriction in cells of the domestic cat

McEwan, W. A., Schaller, T., Ylinen, L. M., Hosie, M. J. , Towers, G. J. and Willett, B. J. (2009) Truncation of TRIM5 in the Feliformia explains the absence of retroviral restriction in cells of the domestic cat. Journal of Virology, 83(16), pp. 8270-8275. (doi: 10.1128/JVI.00670-09) (PMID:19494015) (PMCID:PMC2715776)

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Abstract

TRIM5[alpha] mediates a potent retroviral restriction phenotype in diverse mammalian species. Here, we identify a TRIM5 transcript in cat cells with a truncated B30.2 capsid binding domain and ablated restrictive function which, remarkably, is conserved across the <i>Feliformia</i>. Cat TRIM5 displayed no restriction activity, but ectopic expression conferred a dominant negative effect against human TRIM5[alpha]. Our findings explain the absence of retroviral restriction in cat cells and suggest that disruption of the TRIM5 locus has arisen independently at least twice in the <i>Carnivora</i>, with implications concerning the evolution of the host and pathogen in this taxon.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hosie, Professor Margaret and Willett, Professor Brian
Authors: McEwan, W. A., Schaller, T., Ylinen, L. M., Hosie, M. J., Towers, G. J., and Willett, B. J.
Subjects:Q Science > QR Microbiology > QR355 Virology
S Agriculture > SF Animal culture > SF600 Veterinary Medicine
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Virology
Journal Abbr.:J. Virol.
Publisher:American Society for Microbiology
ISSN:0022-538X
ISSN (Online):1070-6321
Published Online:03 June 2009
Copyright Holders:Copyright © 2009 American Society for Microbiology
First Published:First published in Journal of Virology 83(16):8270-8275
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher.

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
363381Rational Design of a Lentiviral VaccineMargaret HosieMedical Research Council (MRC)G0300387Centre for Virus Research
363381Rational Design of a Lentiviral VaccineMargaret HosieMedical Research Council (MRC)G0300387MVLS III - CENTRE FOR VIRUS RESEARCH
392061Control of Feline Immunodeficiency Virus InfectionMargaret HosieBiotechnology and Biological Sciences Research Council (BBSRC)BB/D008425/1MVLS III - CENTRE FOR VIRUS RESEARCH