McEwan, W. A., Schaller, T., Ylinen, L. M., Hosie, M. J., Towers, G. J. and Willett, B. J. (2009) Truncation of TRIM5 in the Feliformia explains the absence of retroviral restriction in cells of the domestic cat. Journal of Virology, 83(16), pp. 8270-8275. (doi: 10.1128/JVI.00670-09) (PMID:19494015) (PMCID:PMC2715776)
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Abstract
TRIM5[alpha] mediates a potent retroviral restriction phenotype in diverse mammalian species. Here, we identify a TRIM5 transcript in cat cells with a truncated B30.2 capsid binding domain and ablated restrictive function which, remarkably, is conserved across the <i>Feliformia</i>. Cat TRIM5 displayed no restriction activity, but ectopic expression conferred a dominant negative effect against human TRIM5[alpha]. Our findings explain the absence of retroviral restriction in cat cells and suggest that disruption of the TRIM5 locus has arisen independently at least twice in the <i>Carnivora</i>, with implications concerning the evolution of the host and pathogen in this taxon.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Hosie, Professor Margaret and Willett, Professor Brian |
Authors: | McEwan, W. A., Schaller, T., Ylinen, L. M., Hosie, M. J., Towers, G. J., and Willett, B. J. |
Subjects: | Q Science > QR Microbiology > QR355 Virology S Agriculture > SF Animal culture > SF600 Veterinary Medicine |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
Journal Name: | Journal of Virology |
Journal Abbr.: | J. Virol. |
Publisher: | American Society for Microbiology |
ISSN: | 0022-538X |
ISSN (Online): | 1070-6321 |
Published Online: | 03 June 2009 |
Copyright Holders: | Copyright © 2009 American Society for Microbiology |
First Published: | First published in Journal of Virology 83(16):8270-8275 |
Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher. |
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