Truncation of TRIM5 in the Feliformia explains the absence of retroviral restriction in cells of the domestic cat

McEwan, W. A., Schaller, T., Ylinen, L. M., Hosie, M. J., Towers, G. J. and Willett, B. J. (2009) Truncation of TRIM5 in the Feliformia explains the absence of retroviral restriction in cells of the domestic cat. Journal of Virology, 83(16), pp. 8270-8275. (doi: 10.1128/JVI.00670-09) (PMID:19494015) (PMCID:PMC2715776)

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Abstract

TRIM5[alpha] mediates a potent retroviral restriction phenotype in diverse mammalian species. Here, we identify a TRIM5 transcript in cat cells with a truncated B30.2 capsid binding domain and ablated restrictive function which, remarkably, is conserved across the <i>Feliformia</i>. Cat TRIM5 displayed no restriction activity, but ectopic expression conferred a dominant negative effect against human TRIM5[alpha]. Our findings explain the absence of retroviral restriction in cat cells and suggest that disruption of the TRIM5 locus has arisen independently at least twice in the <i>Carnivora</i>, with implications concerning the evolution of the host and pathogen in this taxon.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hosie, Professor Margaret and Willett, Professor Brian
Authors: McEwan, W. A., Schaller, T., Ylinen, L. M., Hosie, M. J., Towers, G. J., and Willett, B. J.
Subjects:Q Science > QR Microbiology > QR355 Virology
S Agriculture > SF Animal culture > SF600 Veterinary Medicine
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation > Centre for Virus Research
Journal Name:Journal of Virology
Journal Abbr.:J. Virol.
Publisher:American Society for Microbiology
ISSN:0022-538X
ISSN (Online):1070-6321
Published Online:03 June 2009
Copyright Holders:Copyright © 2009 American Society for Microbiology
First Published:First published in Journal of Virology 83(16):8270-8275
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher.

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
363381Rational Design of a Lentiviral VaccineMargaret HosieMedical Research Council (MRC)G0300387Centre for Virus Research
363381Rational Design of a Lentiviral VaccineMargaret HosieMedical Research Council (MRC)G0300387MVLS III - CENTRE FOR VIRUS RESEARCH
392061Control of Feline Immunodeficiency Virus InfectionMargaret HosieBiotechnology and Biological Sciences Research Council (BBSRC)BB/D008425/1MVLS III - CENTRE FOR VIRUS RESEARCH