Neutralization of feline immunodeficiency virus by antibodies targeting the V5 loop of Env

Samman, A., Logan, N., McMonagle, E. L., Ishida, T., Mochizuki, M., Willett, B. J. and Hosie, M. J. (2010) Neutralization of feline immunodeficiency virus by antibodies targeting the V5 loop of Env. Journal of General Virology, 91(1), pp. 242-249. (doi:10.1099/vir.0.015404-0)

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Publisher's URL: http://dx.doi.org/10.1099/vir.0.015404-0

Abstract

Neutralising antibodies (NAbs) play a vital role in vaccine-induced protection against infection with feline immunodeficiency virus (FIV). However, little is known about the appropriate presentation of neutralisation epitopes in order to induce NAbs effectively; the majority of the antibodies that are induced are directed against non-neutralising epitopes. Here, we demonstrate that a subtype B strain of FIV, designated NG4, escapes autologous NAbs but may be rendered neutralisation-sensitive following the insertion of two amino acids, Lysine and Threonine, at positions 556-557 in the fifth hypervariable (V5) loop of the envelope glycoprotein (Env). Consistent with the contribution of this motif to virus neutralisation, an additional three subtype B strains retaining both residues at the same position were also neutralised by the NG4 serum and serum from an unrelated cat (TOT1) targeted the same sequence in V5. Moreover, when the V5-loop of subtype B isolate KNG2, an isolate that was moderately resistant to neutralisation by NG4 serum, was mutated to incorporate the K-T motif, the virus was rendered sensitive to neutralisation. These data suggest that even in a polyclonal sera derived from FIV infected cats following natural infection, the primary determinant of virus neutralising activity may be represented by a single, dominant epitope in V5.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hosie, Professor Margaret and Willett, Professor Brian and Samman, Mr Ayman and McMonagle, Mrs Elizabeth and Logan, Miss Nicola
Authors: Samman, A., Logan, N., McMonagle, E. L., Ishida, T., Mochizuki, M., Willett, B. J., and Hosie, M. J.
Subjects:S Agriculture > SF Animal culture > SF600 Veterinary Medicine
Q Science > QR Microbiology > QR355 Virology
S Agriculture > SF Animal culture > SF600 Veterinary Medicine
Q Science > QR Microbiology > QR355 Virology
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of General Virology
Publisher:Society for General Microbiology
ISSN:0022-1317
ISSN (Online):1465-2099
Published Online:23 September 2009
Copyright Holders:Copyright © 2009 Society for General Microbiology
First Published:First published in Journal of General Virology 91(1):242-249
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher.
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
363381Rational Design of a Lentiviral VaccineMargaret HosieMedical Research Council (MRC)G0300387/66515Centre for Virus Research