Survival and differentiation of embryonic neural explants on different biomaterials

Soria, J.M., Martínez Ramos, C., Salmerón-Sánchez, M. , Benavent, V., Campillo Fernández, A., Gómez Ribelles, J.L., García Verdugo, J.M., Monleón Pradas, M. and Barcia, J.A. (2006) Survival and differentiation of embryonic neural explants on different biomaterials. Journal of Biomedical Materials Research Part A, 79A(3), pp. 495-502. (doi:10.1002/jbm.a.30803)

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Abstract

Biomaterials prepared from polyacrylamide, ethyl acrylate (EA), and hydroxyethyl acrylate (HEA) in various blend ratios, methyl acrylate and chitosan, were tested in vitro as culture substrates and compared for their ability to be colonized by the cells migrating from embryonic brain explants. Neural explants were isolated from proliferative areas of the medial ganglionic eminence and the cortical ventricular zone of embryonic rat brains and cultured in vitro on the different biomaterials. Chitosan, poly(methyl acrylate), and the 50% wt copolymer of EA and HEA were the most suitable substrates to promote cell attachment and differentiation of the neural cells among those tested. Immunofluorescence microscopy analysis showed that progenitor cells had undergone differentiation and that the resulting glial and neuronal cells expressed their intrinsic morphological characteristics in culture.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel
Authors: Soria, J.M., Martínez Ramos, C., Salmerón-Sánchez, M., Benavent, V., Campillo Fernández, A., Gómez Ribelles, J.L., García Verdugo, J.M., Monleón Pradas, M., and Barcia, J.A.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Journal of Biomedical Materials Research Part A
ISSN:1549-3296
Published Online:20 June 2006

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