Fibrinogen patterns and activity on substrates with tailored hydroxy density

Rodríguez Hernández, J.C., Rico, P., Moratal, D., Monleón Pradas, M. and Salmerón-Sánchez, M. (2009) Fibrinogen patterns and activity on substrates with tailored hydroxy density. Macromolecular Bioscience, 9(8), pp. 766-775. (doi:10.1002/mabi.200800332)

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Abstract

The influence of the surface fraction of OH groups on fibrinogen (FG) adsorption is investigated in copolymers of ethyl acrylate and hydroxy ethylacrylate. The amount of adsorbed FG, quantified by western-blotting combined with image analysis of the corresponding bands, decreases as the hydrophilicity of the substrate increases. The influence of substrate wettability on FG conformation and distribution is observed by atomic force microscopy (AFM). The most hydrophobic substrate promotes FG fibrillogenesis, which leads to a fibrin-like appearance in the absence of any thrombin. The degree of FG interconnection was quantified by calculating the fractal dimension of the adsorbed protein from image analysis of the AFM results. The biological activity of the adsorbed FG is correlated to cell adhesion on FG-coated substrates.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel
Authors: Rodríguez Hernández, J.C., Rico, P., Moratal, D., Monleón Pradas, M., and Salmerón-Sánchez, M.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Macromolecular Bioscience
ISSN:1616-5187
Published Online:06 May 2009

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