3D adaptive optics in a light sheet microscope

Bourgenot, C., Saunter, C.D., Taylor, J.M. , Girkin, J.M. and Love, G.D. (2012) 3D adaptive optics in a light sheet microscope. Optics Express, 20(12), pp. 13252-13261. (doi:10.1364/OE.20.013252)

Bourgenot, C., Saunter, C.D., Taylor, J.M. , Girkin, J.M. and Love, G.D. (2012) 3D adaptive optics in a light sheet microscope. Optics Express, 20(12), pp. 13252-13261. (doi:10.1364/OE.20.013252)

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Abstract

We report on a single plane illumination microscope (SPIM) incorporating adaptive optics in the imaging arm. We show how aberrations can occur from the sample mounting tube and quantify the aberrations both experimentally and computationally. A wavefront sensorless approach was taken to imaging a green fluorescent protein (GFP) labelled transgenic zebrafish. We show improvements in image quality whilst recording a 3D “z–stack” and show how the aberrations come from varying depths in the fish.

Item Type:Articles
Additional Information:This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://dx.doi.org/10.1364/OE.20.013252. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Taylor, Dr Jonathan
Authors: Bourgenot, C., Saunter, C.D., Taylor, J.M., Girkin, J.M., and Love, G.D.
College/School:College of Science and Engineering > School of Physics and Astronomy
Journal Name:Optics Express
Publisher:Optical Society of America
ISSN:1094-4087
Published Online:29 May 2012
Copyright Holders:Copyright © 2012 Optical Society of America
First Published:First published in Optics Express 20(12):13252-13261
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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