Changes in the serum proteome of canine lymphoma identified by electrophoresis and mass spectrometry

Atherton, M.J., Braceland, M., Fontaine, S. , Waterston, M.M., Burchmore, R.J. , Eadie, S., Eckersall, P.D. and Morris, J.S. (2013) Changes in the serum proteome of canine lymphoma identified by electrophoresis and mass spectrometry. Veterinary Journal, 196(3), pp. 320-324. (doi:10.1016/j.tvjl.2012.12.010)

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Abstract

The serum proteome of canine lymphoma was characterised by one dimensional (1D) serum protein electrophoresis (SPE) on agarose gels, two dimensional (2D) polyacrylamide gel electrophoresis (PAGE) and tandem mass spectrometry (MS). Results were compared with serum proteome data collected previously from the sera of healthy dogs. Twenty-one dogs with high grade multicentric lymphoma had significantly elevated quantities of α2 globulins on 1D SPE. Further separation of the serum proteins was performed on three dogs using a 2D PAGE system. Thirty-six different proteins were identified in 38 bands submitted for MS. Most of the proteins were the same as those previously identified in the sera of healthy dogs. Haptoglobin was identified in the sera of all three dogs with lymphoma and could account for the increased levels of α2 globulins. α2 Macroglobulin, α-antichymotrypsin and inter-α-trypsin inhibitor were also present in dogs with lymphoma. Clusterin, an anti-apoptotic protein, was identified in the serum of one dog with lymphoma. Kininogen, which is present in the sera of healthy dogs, was absent in all three dogs with lymphoma. The 2D electrophoresis technique identified alterations in the serum proteome of dogs with lymphoma and supported previous findings that canine lymphoma has an inflammatory component.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McGill, Mrs Suzanne and Burchmore, Dr Richard and Eckersall, Professor Peter and Fontaine, Ms Samantha and Atherton, Mr Matthew and Waterston, Miss Mary and Morris, Professor Joanna and Braceland, Mr Mark
Authors: Atherton, M.J., Braceland, M., Fontaine, S., Waterston, M.M., Burchmore, R.J., Eadie, S., Eckersall, P.D., and Morris, J.S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Veterinary Journal
Publisher:Elsevier Ltd
ISSN:1090-0233
Published Online:29 January 2013

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