Schistosoma mansoni cercariae experience influx of macromolecules during skin penetration

Thornhill, J., Coelho, P.M.Z., McVeigh, P., Maule, A., Jurberg, A.D. and Kusel, J.R. (2009) Schistosoma mansoni cercariae experience influx of macromolecules during skin penetration. Parasitology, 136(11), pp. 1257-1267. (doi: 10.1017/S0031182009990692)

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Publisher's URL: http://dx.doi.org/10.1017/S0031182009990692

Abstract

We have observed that when cercariae penetrate the skin of mice, there is influx into their tissues of Lucifer Yellow and certain labelled molecules of up to 20 kDa molecular weight. This observation was made using a variety of fluorescent membrane-impermeant compounds injected into the skin before the application of cercariae. This unexpected phenomenon was investigated further by transforming cercariae in vitro in the presence of the membrane-impermeant compounds and examining the distribution by microscopy. In schistosomula derived from this procedure, the nephridiopore and surface membrane were labelled while the pre- and post-acetabular glands were not labelled. The region associated with the oesophagus within the pharyngeal muscle clearly contained the fluorescent molecules, as did the region adjacent to the excretory tubules and the germinal mass. We used cercariae stained with carmine to aid identification of regions labelled with Lucifer Yellow. Although the mechanism of this influx is unclear, the observation is significant. From it, we can suggest an hypothesis that, during skin penetration, exposure of internal tissues of the parasite to external macromolecules represents a novel host-parasite interface

Item Type:Articles
Keywords:Cercaria, fluorescent dextrans, in vitro transformation, Lucifer Yellow, propidium iodide, schistosomulum, skin penetration
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Kusel, Dr John and Thornhill, Mrs Joyce
Authors: Thornhill, J., Coelho, P.M.Z., McVeigh, P., Maule, A., Jurberg, A.D., and Kusel, J.R.
Subjects:Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
College/School:College of Medical Veterinary and Life Sciences
Journal Name:Parasitology
Publisher:Cambridge University Press
ISSN:0031-1820
ISSN (Online):1469-8161
Published Online:03 August 2009
Copyright Holders:Copyright © 2009 Cambridge University Press
First Published:First published in Parasitology 136(11):1257-1267
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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