Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms

Andenmatten, N., Egarter, S., Jackson, A.J., Jullien, N., Herman, J.-P. and Meissner, M. (2013) Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms. Nature Methods, 10, pp. 125-127. (doi:10.1038/nmeth.2301)

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Abstract

We established a conditional site-specific recombination system based on dimerizable Cre recombinase−mediated recombination in the apicomplexan parasite Toxoplasma gondii. Using a new single-vector strategy that allows ligand-dependent, efficient removal of a gene of interest, we generated three knockouts of apicomplexan genes considered essential for host-cell invasion. Our findings uncovered the existence of an alternative invasion pathway in apicomplexan parasites.

Item Type:Articles
Keywords:Toxoplasma gondii, invasion, diCre, inducible knock out, myoA, mic2
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Meissner, Professor Markus and Andenmatten, Ms Nicole and Jackson, Dr Allison
Authors: Andenmatten, N., Egarter, S., Jackson, A.J., Jullien, N., Herman, J.-P., and Meissner, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Research Group:Meissner
Journal Name:Nature Methods
Journal Abbr.:Nat Meth
Publisher:Nature America Inc
ISSN:1548-7091
Published Online:23 December 2012

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