Andenmatten, N., Egarter, S., Jackson, A.J., Jullien, N., Herman, J.-P. and Meissner, M. (2013) Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms. Nature Methods, 10, pp. 125-127. (doi: 10.1038/nmeth.2301)
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Abstract
We established a conditional site-specific recombination system based on dimerizable Cre recombinase−mediated recombination in the apicomplexan parasite Toxoplasma gondii. Using a new single-vector strategy that allows ligand-dependent, efficient removal of a gene of interest, we generated three knockouts of apicomplexan genes considered essential for host-cell invasion. Our findings uncovered the existence of an alternative invasion pathway in apicomplexan parasites.
Item Type: | Articles |
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Keywords: | Toxoplasma gondii, invasion, diCre, inducible knock out, myoA, mic2 |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Meissner, Professor Markus and Andenmatten, Ms Nicole and Jackson, Dr Allison |
Authors: | Andenmatten, N., Egarter, S., Jackson, A.J., Jullien, N., Herman, J.-P., and Meissner, M. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Research Group: | Meissner |
Journal Name: | Nature Methods |
Journal Abbr.: | Nat Meth |
Publisher: | Nature America Inc |
ISSN: | 1548-7091 |
Published Online: | 23 December 2012 |
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