Molecular cloning and localization of a calpain-like protease from the abdominal muscle of Norway lobster Nephrops norvegicus

Gornik, S.G., Westrop, G.D., Coombs, G.H. and Neil, D.M. (2010) Molecular cloning and localization of a calpain-like protease from the abdominal muscle of Norway lobster Nephrops norvegicus. Molecular Biology Reports, 37(4), pp. 2009-2019. (doi: 10.1007/s11033-009-9652-x) (PMID:19642014)

Abstract

Calpains are ubiquitous cysteine-proteases found in many, if not all, living organisms and their roles within these organisms are diverse, ranging from the mediation of cytoskeletal remodeling to the regulation of gene expression. In crustaceans calpains have so far been shown to be important mainly during moulting and growth. In the present study we report the expression of a calpain in the abdominal muscle of Norway lobster (<i>Nephrops norvegicus</i>) using degenerate primer, rapid amplification of cDNA ends (5'-3'-RACE), reverse transcriptase-PCR and RNA in situ hybridization approaches. The full-length mRNA sequence (2,774bp) was found to include an open reading frame (bp 225-1,940) encoding a 572 amino acid polypeptide with a predicted mass of 65.9kDa and a predicted pI of 5.17. The calpain was found to be an arthropod M-class calpain homologue to <i>Homarus americanus</i> Calpain M (Ha-CalpM) and has thus been termed <i>Nephrops norvegicus</i> calpain M (Nn-CalpM). When its expression pattern in abdominal muscle of adult intermoult <i>Nephrops norvegicus</i> was investigated an exclusive expression in a thin layer of connective tissue cells surrounding muscle fibres was found. This localization suggests a role in tenderizing connective tissue networks during growth and moulting.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Neil, Professor Douglas and Coombs, Professor Graham
• Authors: Gornik, S.G., Westrop, G.D., Coombs, G.H., and Neil, D.M.
• Subjects: Q Science > QH Natural history > QH301 Biology
• College/School: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: Molecular Biology Reports
• Publisher: Springer Netherlands
• ISSN: 0301-4851
• ISSN (Online): 1573-4978
• Published Online: 30 July 2009