Crystallization and preliminary structural studies of champedak galactose-binding lectin

Gabrielsen, M., Riboldi-Tunnicliffe, A., Abdul-Rahman, P.S., Mohamed, E., Ibrahim, W.I.W., Hashim, O.H., Isaacs, N.W. and Cogdell, R.J. (2009) Crystallization and preliminary structural studies of champedak galactose-binding lectin. Acta Crystallographica. Section F: Structural Biology and Crystallization Communications, 65(9), pp. 895-897. (doi: 10.1107/S1744309109029303)

Abstract

Galactose-binding lectin from champedak (Artocarpus integer) consists of two chains: [alpha] and [beta] (133 and 21 amino acids, respectively). It has been shown to recognize and bind to carbohydrates involved in IgA and C1 inhibitor molecules. The protein was purified and crystallized at 293 K. Crystals were observed in two space groups, P2₁ and P2₁2₁2, and diffracted to 1.65 and 2.6 Å, respectively.

• Item Type: Articles
• Keywords: Lectins, artocarpus integer, champedak, galactose binding
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Cogdell, Professor Richard and Gabrielsen, Dr Mads and Isaacs, Professor Neil and Riboldi-Tunnicliffe, Dr Alan
• Authors: Gabrielsen, M., Riboldi-Tunnicliffe, A., Abdul-Rahman, P.S., Mohamed, E., Ibrahim, W.I.W., Hashim, O.H., Isaacs, N.W., and Cogdell, R.J.
• Subjects: Q Science > QH Natural history > QH345 Biochemistry
• College/School: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences; College of Science and Engineering > School of Chemistry
• Journal Name: Acta Crystallographica. Section F: Structural Biology and Crystallization Communications
• Publisher: Wiley-Blackwell Publishing, Inc.
• ISSN: 1744-3091
• ISSN (Online): 1744-3091