2D-DIGE proteomic analysis of mesenchymal stem cell cultured on the elasticity-tunable hydrogels

Kuboki, T., Kantawong, F., Burchmore, R. , Dalby, M.J. and Kidoaki, S. (2012) 2D-DIGE proteomic analysis of mesenchymal stem cell cultured on the elasticity-tunable hydrogels. Cell Structure and Function, 37(2), pp. 127-139. (doi:10.1247/csf.12012)

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Abstract

The present study focuses on mechanotransduction in mesenchymal stem cells (MSCs) in response to matrix elasticity. By using photocurable gelatinous gels with tunable stiffness, proteomic profiles of MSCs cultured on tissue culture plastic, soft (3 kPa) and stiff (52 kPa) matrices were deciphered using 2-dimensional differential in-gel analysis (2D-DIGE). The DIGE data, tied to immunofluorescence, indicated abundance and organization changes in the cytoskeletonal proteins as well as differential regulation of important signaling-related proteins, stress-responsing proteins and also proteins involved in collagen synthesis. The major CSK proteins including actin, tubulin and vimentin of the cells cultured on the gels were remarkably changed their expressions. Significant down-regulation of α-tubulin and β-actin can be observed on gel samples in comparison to the rigid tissue culture plates. The expression abundance of vimentin appeared to be highest in the MSCs cultured on hard gels. These results suggested that the substrate stiffness significantly affects expression balances in cytoskeletal proteins of MSCs with some implications to cellular tensegrity.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Burchmore, Dr Richard and Dalby, Professor Matthew
Authors: Kuboki, T., Kantawong, F., Burchmore, R., Dalby, M.J., and Kidoaki, S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Cell Structure and Function
ISSN:0386-7196
Published Online:31 August 2012

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