Scar/WAVE3 contributes to motility and plasticity of lamellipodial dynamics but not invasion in three dimensions

Spence, H.J., Timpson, P., Tang, H.R., Insall, R.H. and Machesky, L.M. (2012) Scar/WAVE3 contributes to motility and plasticity of lamellipodial dynamics but not invasion in three dimensions. Biochemical Journal, 448(1), pp. 35-42. (doi:10.1042/BJ20112206)

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Abstract

The Scar (suppressor of cAMP receptor)/WAVE [WASP (Wiskott–Aldrich syndrome protein) verprolin homologous] complex plays a major role in the motility of cells by activating the Arp2/3 complex, which initiates actin branching and drives protrusions. Mammals have three Scar/WAVE isoforms, which show some tissue-specific expression, but their functions have not been differentiated. In the present study we show that depletion of Scar/WAVE3 in the mammalian breast cancer cells MDA-MB-231 results in larger and less dynamic lamellipodia. Scar/WAVE3-depleted cells move more slowly but more persistently on a two-dimensional matrix and they typically only show one lamellipod. However, Scar/WAVE3 appears to have no role in driving invasiveness in a three-dimensional Matrigel™ invasion assay or a three-dimensional collagen invasion assay, suggesting that lamellipodial persistence as seen in two-dimensions is not crucial in three-dimensional environments.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Machesky, Professor Laura and Insall, Professor Robert and Timpson, Dr Paul and Tang, Mr Hao and Spence, Dr Heather
Authors: Spence, H.J., Timpson, P., Tang, H.R., Insall, R.H., and Machesky, L.M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Biochemical Journal
Publisher:Portland Press Ltd.
ISSN:0264-6021
ISSN (Online):1470-8728
Published Online:21 August 2012

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