Isoelectric focusing and reverse immunoblotting of autoantibodies against high molecular weight antigens

Stott, D.I. and McLearie, J. (1986) Isoelectric focusing and reverse immunoblotting of autoantibodies against high molecular weight antigens. Immunological Investigations, 15(2), pp. 113-122. (doi:10.3109/08820138609094137)

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Abstract

A method for the clonal analysis of antibodies against high M.Wt. antigens is described. It involves isoelectric focusing, electrophoretic transfer of the focused antibodies to a nitrocellulose membrane and detection of the membrane-bound antibodies by overlay with radiolabelled antigen (reverse immunoblotting). The application of this technique to the clonal analysis of autoantibodies against thyroglobulin and DNA is described.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Stott, Professor David
Authors: Stott, D.I., and McLearie, J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Immunological Investigations
ISSN:0882-0139

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