Calaminus, S. D.J., Guitart, A. V., Sinclair, A., Schachtner, H., Watson, S. P., Holyoake, T. L., Kranc, K. R. and Machesky, L. M. (2012) Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. PLoS ONE, 7(12), e51361. (doi: 10.1371/journal.pone.0051361) (PMID:23300543) (PMCID:PMC3531453)
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Abstract
The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs), including the most primitive fraction containing the long-term repopulating HSCs. Consequently, we demonstrate that Pf4-Cre activity is not megakaryocyte lineage-specific but extends to other myeloid and lymphoid lineages at significant levels between 15–60%. Finally, we show for the first time that Pf4 transcripts are present in adult HSCs and primitive hematopoietic progenitor cells. These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage.
Item Type: | Articles |
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Additional Information: | A correction to this article is available at https://doi.org/10.1371/annotation/4c610387-c7f0-4330-b772-30ed52a07547. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Calaminus, Dr Simon and Holyoake, Professor Tessa and Machesky, Professor Laura and Sinclair, Miss Amy and Guitart, Dr Amelie and Kranc, Professor Kamil |
Authors: | Calaminus, S. D.J., Guitart, A. V., Sinclair, A., Schachtner, H., Watson, S. P., Holyoake, T. L., Kranc, K. R., and Machesky, L. M. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
Journal Name: | PLoS ONE |
Publisher: | Public Library of Science |
ISSN: | 1932-6203 |
ISSN (Online): | 1932-6203 |
Copyright Holders: | Copyright © 2012 The Authors |
First Published: | First published in PloS ONE 7(12):e51361 |
Publisher Policy: | Reproduced under a Creative Commons License |
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