A gel transfer tank for immunoblotting and its application for analysis of nuclear protein antigens

Stott, D.I., McLearie, J. and Marsden, H.S. (1985) A gel transfer tank for immunoblotting and its application for analysis of nuclear protein antigens. Analytical Biochemistry, 149(2), pp. 454-460. (doi:10.1016/0003-2697(85)90597-4)

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Abstract

The design of a gel transfer tank for immunoblotting is described. It is simple and cheap to make, provides a uniform field and uniform transfer over the whole area of the gel, and can easily be adapted for use with any size of gel. It has been used for transfer of proteins from both sodium dodecyl sulfate-polyacrylamide and isoelectric focusing gels to nitrocellulose membranes and its application to the analysis of nuclear proteins is described.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Stott I, Professor David
Authors: Stott, D.I., McLearie, J., and Marsden, H.S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Analytical Biochemistry
ISSN:0003-2697

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