Nonhistone chromatin proteins of B-lymphocytes stimulated by lipopolysaccharide. Two-dimensional gel electrophoresis analysis of proteins synthesized and phosphorylated during the initiation of lymphocyte differentiation

Stott, D.I. (1982) Nonhistone chromatin proteins of B-lymphocytes stimulated by lipopolysaccharide. Two-dimensional gel electrophoresis analysis of proteins synthesized and phosphorylated during the initiation of lymphocyte differentiation. Biochimica et Biophysica Acta: General Subjects, 699(3), pp. 247-254.

Full text not currently available from Enlighten.

Abstract

Synthesis and phosphorylation of nonhistone chromatin and nucleoplasmic proteins during the first 24 h of activation of mouse B-lymphocytes by the B-cell mitogen lipopolysaccharide have been studied by two-dimensional gel electrophoretic analysis. Although little change occurs in the nucleoplasmic proteins, it has been shown that the incorporation of [35S]methionine into nonhistone chromatin proteins is selectively stimulated. The degree of stimulation and the kinetics of synthesis are characteristic for each individual protein; some proteins exhibit increased incorporation only 4 h after addition of mitogen, while others are synthesized de novo between 8 and 24 h. After 72 h stimulation, the majority of nonhistone chromatin protein synthesis occurs in the highly differentiated lymphoblasts and plasma cells actively secreting IgM, very little synthesis taking place in the small lymphocytes. Analysis of nuclear proteins from lymphocytes stimulated for 2 h showed no selective stimulation of phosphorylation. These observations suggest that nonhistone chromatin proteins play an important role in the regulation of gene expression in B-lymphocytes.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Stott I, Professor David
Authors: Stott, D.I.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Biochimica et Biophysica Acta: General Subjects
Publisher:Elsevier BV
ISSN:0304-4165

University Staff: Request a correction | Enlighten Editors: Update this record