Smith, G.W.G., McNeil, J., Ramage, G. and Smith, A.J. (2012) In vitro evaluation of cleaning efficacy of detergents recommended for use on dental instruments. American Journal of Infection Control, 40(9), e255-e259. (doi: 10.1016/j.ajic.2012.05.009)
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Abstract
<p>Background: The cleaning stage of instrument decontamination processes is a critical control point, and removal of protein deposits is used as a marker of cleaning efficacy. An important factor is the choice of cleaning solution especially in the absence of any defined standards for detergent effectiveness.</p> <p>Methods: Following method validation, stainless steel tokens were inoculated with reconstituted citrated blood and added to a 24-multiwell plate and immersed in different cleaning solutions for 5 minutes, agitated at 25 (20°) tilts/min at 22°C and at the manufacturers' recommended temperatures. Desorbed protein was measured using the bicinchoninic acid assay.</p> <p>Results: From a starting concentration with a median of 3,700 μg of blood protein of all solutions tested, alkaline detergent (Haemo-sol) removed the largest proportion of protein (median, 2,070 μg), and surgical handwash removed the least protein (median, 0 μg). Reverse osmosis water demonstrated useful blood-removing properties with a median of 1,421 μg.</p> <p>Conclusion: The cleaning system we utilized is a simple, inexpensive method to compare the cleaning efficacies of detergents and may be used as a first stage in benchmarking cleaning efficacy of detergents. Not all solutions used in cleaning dental instruments are efficacious at removal of blood.</p>
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Smith, Professor Andrew and Ramage, Professor Gordon |
Authors: | Smith, G.W.G., McNeil, J., Ramage, G., and Smith, A.J. |
College/School: | College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School |
Journal Name: | American Journal of Infection Control |
ISSN: | 0196-6553 |
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