Protocol: optimised electrophyiological analysis of intact guard cells from arabidopsis

Chen, Z.-H., Eisenach, C., Xu, X.Q., Hills, A. and Blatt, M.R. (2012) Protocol: optimised electrophyiological analysis of intact guard cells from arabidopsis. Plant Methods, 8(15), (doi:10.1186/1746-4811-8-15)

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Abstract

Genetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is not matched by accessible tools for quantitative analysis of transport in the intact cell. We have developed a reliable set of procedures for voltage clamp analysis of guard cells from Arabidopsis leaves. These procedures greatly simplify electrophysiological recordings, extending the duration of measurements and scope for analysis of the predominant K+ and anion channels of intact stomatal guard cells to that achieved previously in work with Vicia and tobacco guard cells.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hills, Mr Adrian and Blatt, Professor Michael and Eisenach, Ms Cornelia
Authors: Chen, Z.-H., Eisenach, C., Xu, X.Q., Hills, A., and Blatt, M.R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Plant Methods
Publisher:BioMed Central
ISSN:1746-4811
Published Online:06 May 2012
Copyright Holders:Copyright © 2012 The Authors
First Published:First published in Plant Methods 8:15
Publisher Policy:Reproduced under a Creative Commons License

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