Chen, Z.-H., Eisenach, C., Xu, X.Q., Hills, A. and Blatt, M.R. (2012) Protocol: optimised electrophyiological analysis of intact guard cells from arabidopsis. Plant Methods, 8(15), (doi: 10.1186/1746-4811-8-15)
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Abstract
Genetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is not matched by accessible tools for quantitative analysis of transport in the intact cell. We have developed a reliable set of procedures for voltage clamp analysis of guard cells from Arabidopsis leaves. These procedures greatly simplify electrophysiological recordings, extending the duration of measurements and scope for analysis of the predominant K+ and anion channels of intact stomatal guard cells to that achieved previously in work with Vicia and tobacco guard cells.
| Item Type: | Articles |
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| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Hills, Mr Adrian and Blatt, Professor Michael and Eisenach, Ms Cornelia |
| Authors: | Chen, Z.-H., Eisenach, C., Xu, X.Q., Hills, A., and Blatt, M.R. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | Plant Methods |
| Publisher: | BioMed Central |
| ISSN: | 1746-4811 |
| Published Online: | 06 May 2012 |
| Copyright Holders: | Copyright © 2012 The Authors |
| First Published: | First published in Plant Methods 8:15 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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