Physical and genetic mapping of the protein A gene in the chromosome of Staphylococcus aureus 8325-4

Patel, A.H. , Foster, T.J. and Pattee, P.A. (1989) Physical and genetic mapping of the protein A gene in the chromosome of Staphylococcus aureus 8325-4. Journal of General Microbiology, 135(7), pp. 1799-807. (doi:10.1099/00221287-135-7-1799)

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Abstract

The gene coding for protein A (spa) has been mapped close to nov on the genetic map of the chromosome of Staphylococcus aureus 8325-4. A rapid mapping procedure has been developed which first allowed the region of the chromosome carrying the spa gene to be identified by blot +hybridization of large DNA fragments which had been separated by pulsed-field gel electrophoresis. Restriction endonuclease SmaI fragment G was shown to carry the spa gene. An insertion mutation in spa was constructed by in vitro insertion of a fragment of DNA expressing resistance to kanamycin and neomycin. A spa::Kan(r)Neo(r) mutation was isolated in S. aureus 8325-4 by allele replacement. This provided a selectable marker which allowed the spa gene to be mapped by transformation analysis.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Patel, Professor Arvind
Authors: Patel, A.H., Foster, T.J., and Pattee, P.A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of General Microbiology
Publisher:Society for General Microbiology
ISSN:0022-1287

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