The DNA binding domain of the Varicella-zoster virus gene 62 protein interacts with multiple sequences which are similar to the binding site of the related protein of herpes simplex virus type 1

Tyler, J.K. and Everett, R.D. (1993) The DNA binding domain of the Varicella-zoster virus gene 62 protein interacts with multiple sequences which are similar to the binding site of the related protein of herpes simplex virus type 1. Nucleic Acids Research, 21(3), pp. 513-522. (doi:10.1093/nar/21.3.513)

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Abstract

Varicella-zoster virus gene 62 encodes a protein with predicted Mr of 140,000D (VZV 140k) that shares extensive predicted amlno add sequence homology with the major Immediate early (IE) transcrlptlonal regulator protein of herpes simplex virus type 1 (HSV-1) Vmw175. The integrity of highly conserved region 2 is essential for the DNA binding and transcrlptlonal regulatory functions of Vmw175. Similarly, an Insertion mutation in region 2 (codons 468-641) of 140k eliminates the transcrlptlonal repression and activation functions of this protein. We have expressed a fragment of 140k which encompasses region 2 as a non-fusion polypeptlde in bacteria. This 140k DNA binding domain peptlde (codons 417-646) binds to numerous DNA sequences throughout the VZV gene 62 promoter region. It induces multiple regions of protection from DNase I digestion, flanked by sites of DNase I hypersensltivity. Several of the sites recognized can be considered to be divergent forms of the consensus sequence which is recognized by Vmw175. However, by use of a panel of mutagenlzed probe fragments, we found that the 140k DNA binding domain was less sequence-specific than Vmw175 In Its Interactions with DNA. Consistent with this, the homologous Vmw175 DNA binding domain, and also Intact Vmw175, recognize the gene 62 binding sites much less efficiently than the 140k DNA binding domain. Also In contrast to the situation with Vmw175, the140k DNA binding domain failed to induce DNA bending when occupying the binding sites In its own promoter. Deletion analysis has mapped the minimal DNA binding domain of the VZV 140k protein, as measured in gel retardation analysis, to lie within residues 472 to 633. The differences in binding characteristics of the DNA binding domains of the homologous VZV 140k and HSV-1 Vmw175 IE proteins may account for the subtle differences In their regulatory activities in transfection assays and during virus growth In tissue culture.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Everett, Professor Roger
Authors: Tyler, J.K., and Everett, R.D.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Nucleic Acids Research
Publisher:Oxford University Press
ISSN:0305-1048
ISSN (Online):1362-4962
Copyright Holders:Copyright © 1993 Oxford University Press
First Published:First published in Nucleic Acids Research 21(3):513-522
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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