Pizer, L.I., Everett, R.D., Tedder, D.G., Elliott, M. and Litman, B. (1991) Nucleotides within both proximal and distal parts of the consensus sequence are important for specific DNA recognition by the herpes simplex virus regulatory protein ICP4. Nucleic Acids Research, 19(3), pp. 477-483. (doi: 10.1093/nar/19.3.477)
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Abstract
The herpes simplex virus type 1 regulatory protein ICP4 is a sequence specific DNA binding protein which associates with a number of different sites, some of which include the consensus ATCGTCnnnnYCGRC. In order to investigate the involvement in DNA binding of conserved bases within the consensus, we have synthesised a family of mutant oligonucleotides and tested their ability to form a complex with ICP4. We have also compared the binding specificities of bacterially expressed fragments of ICP4 which include the DNA binding domain. Mutation of most (but not all) bases In the proximal part of the consensus greatly reduced binding by ICP4, as did a mutation affecting the distal part. Most (but not all) G residues identified in methylatlon interference assays were required for efficient binding. While a bacterially expressed ICP4 peptide encompassing amino acid residues 252 – 523 bound to DNA with a specificity similar to that of the whole protein, a shorter protein (residues 275 – 523) had a slightly relaxed DNA binding specificity.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Everett, Professor Roger |
Authors: | Pizer, L.I., Everett, R.D., Tedder, D.G., Elliott, M., and Litman, B. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Nucleic Acids Research |
Publisher: | Oxford University Press |
ISSN: | 0305-1048 |
ISSN (Online): | 1362-4962 |
Copyright Holders: | Copyright © 1991 Oxford University Press |
First Published: | First published in Nucleic Acids Research 19(3):477-483 |
Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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