Johnson, P.A. and Everett, R.D. (1986) The control of herpes simplex virus type-1 late gene transcription: a ‘TATA-box’/cap site region is sufficient for fully efficient regulated activity. Nucleic Acids Research, 14(21), pp. 8247-8264. (doi: 10.1093/nar/14.21.8247)
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Abstract
The transcriptional programme of herpes simplex virus type 1 (HSV-1) is organised into three principle phases; immediate-early (IE), early (E) and late. The appearance of IE gene products provides the switch for E transcription. Abundant expression of late genes requires viral DKA replication. There is some overlap between E and late genes according to their degree of dependence on DNA replication. The pattern of expression of gene USll is regulated with ‘true-late’ kinetics (Johnson et al., 1986) . In a transient assay system, regulation of a plasmid-borne USll promoter mimics its viral counterpart, and has a similar dependence on DNA replication for abundant expression. Using plasmids which contain a functional HSV-1 origin of replication (ORIs), we have identified the sequence requirements for the expression of late genes. All DNA sequence elements necessary for fully efficient regulated expression of USll lie within 31 bp of the RNA cap sites; therefore it appears that a late gene promoter consists only of a proximal ‘TATA-box’ and cap-site region. We tested this hypothesis by removing the distal upstream region of the gD promoter (which is required for its normal regulation as an early promoter) and linking this truncated promoter to ORIs This resulted in the conversion of gD promoter regulation to late gene kinetics during virus superinfection. The implications of these results for the mechanisms of HSV gene regulation are discussed.
| Item Type: | Articles |
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| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Everett, Professor Roger |
| Authors: | Johnson, P.A., and Everett, R.D. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Nucleic Acids Research |
| Publisher: | Oxford University Press |
| ISSN: | 0305-1048 |
| ISSN (Online): | 1362-4962 |
| Copyright Holders: | Copyright © 1986 IRL Press Limited |
| First Published: | First published in Nucleic Acids Research 14(21):8247-8264 |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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