High level expression and purification of herpes simplex virus type 1 immediate early polypeptide Vmw110

Everett, R.D., Orr, A. and Elliott, M. (1991) High level expression and purification of herpes simplex virus type 1 immediate early polypeptide Vmw110. Nucleic Acids Research, 19(22), pp. 6155-6161. (doi: 10.1093/nar/19.22.6155)

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Abstract

Herpes simplex virus type 1 (HSV-1) encodes five immediate early (IE) polypeptides. This paper reports the construction of a baculovirus vector which expresses large amounts of Vmw110, the product of IE gene 1. The expressed protein has been purified to near homogeneity and has a mobility on SDS polyacrylamide gels identical to that of Vmw110 produced during HSV-1 infection. Characterisation of its properties indicated that it forms dimers and perhaps higher order oligomers in solution and that the purified protein binds to both single stranded and double stranded calf thymus DNA cellulose columns. However, filter binding experiments were unable to detect any stable association of Vmw110 with DNA in solution.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Everett, Professor Roger and Orr, Mrs Anne
Authors: Everett, R.D., Orr, A., and Elliott, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Nucleic Acids Research
Publisher:Oxford University Press
ISSN:0305-1048
ISSN (Online):1362-4962
Copyright Holders:Copyright © 1991 Oxford University Press
First Published:First published in Nucleic Acids Research 19(22):6155-6161
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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