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DNA replication of bacteriophage T5. 1. fractionation of intracellular T5 DNA by agarose gel electrophoresis

Everett, R.D., and Lunt, M.R. (1980) DNA replication of bacteriophage T5. 1. fractionation of intracellular T5 DNA by agarose gel electrophoresis. Journal of General Virology, 47 (1). pp. 123-132. ISSN 0022-1317 (doi:10.1099/0022-1317-47-1-123)

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Publisher's URL: http://dx.doi.org/10.1099/0022-1317-47-1-123

Abstract

Two forms of DNA may be isolated from gently lysed T5-infected bacteria. In neutral sucrose gradients a fast sedimenting form (fsf) is distinguishable from a slow sedimenting form (ssf) which moves at a rate similar to that of DNA extracted from mature T5 virus particles. A method of agarose gel electrophoresis is described which gives complete separation of the slow sedimenting from the fast sedimenting form. The DNA was extracted from the agarose using potassium iodide and isopycnic centrifugation; samples of the slow sedimenting form prepared by these methods were suitable for detailed structural studies.

Item Type:Article
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Everett, Prof Roger
Authors: Everett, R.D., and Lunt, M.R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of General Virology
Journal Abbr.:J. Gen. Virol.
Publisher:Society for General Microbiology
ISSN:0022-1317
ISSN (Online):1465-2099

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