The repeated GC-rich motifs upstream from the TATA box are important elements of the SV40 early promoter

Everett, R.D., Baty, D. and Chambon, P. (1983) The repeated GC-rich motifs upstream from the TATA box are important elements of the SV40 early promoter. Nucleic Acids Research, 11(8), pp. 2447-2464. (doi:10.1093/nar/11.8.2447)

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Publisher's URL: http://dx.doi.org/10.1093/nar/11.8.2447

Abstract

We have constructed deletion and point mutations within the Simian virus 40 (SV40) early promoter region which contains two tandemly repeated 21 bp sequences and a related 22 bp sequence (the “upstream” 21 bp repeat region). After transfection into permissive CV-1 cells and non-permissive mouse 3T3-4E cells, the effect of the mutations on early gene expression was studied by measuring T-antigen production, using indirect immunofluoresence. Our results demonstrate that the 21 bp repeat region, and in particular the six GC-rich motifs 5′-CCGCCC-3′ which are repeated in this region constitute an important element of the SV40 early promoter. Surprisingly, we found that the requirement for the 21 bp repeat region for early gene expression was partially fulfilled even when it was in the inverted orientation.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Everett, Professor Roger
Authors: Everett, R.D., Baty, D., and Chambon, P.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Nucleic Acids Research
Journal Abbr.:Nucl. Acids Res.
ISSN:0305-1048
ISSN (Online):1362-4962

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