DNA sequence elements required for regulated expression of the HSV-1 glycoprotein D gene lie within 83 bp of the RNA capsites

Everett, R.D. (1983) DNA sequence elements required for regulated expression of the HSV-1 glycoprotein D gene lie within 83 bp of the RNA capsites. Nucleic Acids Research, 11(19), pp. 6647-6666. (doi:10.1093/nar/11.19.6647)

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Publisher's URL: http://dx.doi.org/10.1093/nar/11.19.6647

Abstract

The genes of Herpes simplex virus type 1 (HSV-1) are classified into three temporally regulated groups. The Immediate-Early (IE) genes are transcribed first by the pre-existing transcription apparatus of the cell. The Early genes are transcribed only after IE-gene expression, and finally the Late genes are activated. The control of transcription of the HSV-1 glycoprotein D (gD) gene (an Early function) was studied by quantitative S1 mapping of RNA produced in HSV-1 infected HeLa cells after short-term transfection experiments using plasmids containing the gD promoter linked to the rabbit β-globin gene. The viral promoter in the plasmid was activated in the same way as that in the virus itself; the RNA showed a similar time-course of appearance, dependence on prior IE-gene expression and pattern of RNA cap-sites. Deletion analysis showed that the DNA sequences necessary for Early promoter activation lie within 83 bp of the RNA cap-sites in this instance. Surprisingly, a plasmid-borne β-globin promoter was also activated by HSV-1 infection. The mechanism of this activation, and DNA sequence similarities between the promoters of HSV-1 Early and rabbit β-globin genes are discussed.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Everett, Professor Roger
Authors: Everett, R.D.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Nucleic Acids Research
Journal Abbr.:Nucl. Acids Res.
ISSN:0305-1048
ISSN (Online):1362-4962

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