Enlighten
Research publications by members of the University of Glasgow
home > services > Enlighten

A herpes simplex virus type 1 recombinant with both copies of the Vmw175 coding sequences replaced by the homologous varicella-zoster virus open reading frame

Disney, G.H., and Everett, R.D. (1990) A herpes simplex virus type 1 recombinant with both copies of the Vmw175 coding sequences replaced by the homologous varicella-zoster virus open reading frame. Journal of General Virology, 71 (11). pp. 2681-2689. ISSN 0022-1317 (doi:10.1099/0022-1317-71-11-2681)

Full text not currently available from Enlighten.

Publisher's URL: http://dx.doi.org/10.1099/0022-1317-71-11-2681

Abstract

Varicella-zoster virus (VZV) gene 62 encodes a protein with a predicted Mr of 140000 (VZV 140K) that shares considerable amino acid homology with the immediate early (IE) regulatory protein Vmw175 of herpes simplex virus type 1 (HSV-1) and is believed to be its functional equivalent. We have tested this hypothesis by insertion of VZV gene 62 (expressed from the HSV-1 IE3 promoter) into both IE3 gene loci in the short region repeats of the HSV-1 genome. The parent virus used for this manipulation was D30EBA, which is a variant of HSV-1 from which the majority of the Vmw175 coding sequences have been deleted. Like other HSV-1 viruses lacking Vmw175 function, D30EBA is able to grow only in cell lines which express Vmw175 constitutively. The resulting recombinant virus, HSV-140, is able to propagate (but unable to form obvious plaques) on normal cell lines. The properties of HSV-140 were studied by monitoring the time course of polypeptide expression and DNA replication during normal infection. We found that at high multiplicity HSV-140 synthesized apparently normal amounts of many viral polypeptides but that the expression of certain late genes was reduced; this slight defect may be related to less efficient DNA replication by HSV-140. At low multiplicity HSV-140 expressed viral proteins inefficiently. Surprisingly, VZV 140K was produced in large amounts at later times of a normal infection, indicating that the polypeptide fails to autoregulate the IE3 promoter. The results strongly suggest that VZV 140K is able to perform most of the functions of Vmw175 during growth of HSV-1, but that differences in detail lead to less efficient virus growth.

Item Type:Article
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Everett, Prof Roger
Authors: Disney, G.H., and Everett, R.D.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of General Virology
Journal Abbr.:J. Gen. Virol.
Publisher:Society for General Microbiology
ISSN:0022-1317
ISSN (Online):1465-2099

University Staff: Request a correction | Enlighten Editors: Update this record