Activation of gene expression by human herpesvirus 6 is reporter gene-dependent

Campbell, M.E.M., McCorkindale, S., Everett, R.D. and Onions, D.E. (1991) Activation of gene expression by human herpesvirus 6 is reporter gene-dependent. Journal of General Virology, 72(5), pp. 1123-1130. (doi: 10.1099/0022-1317-72-5-1123)

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Infection with human herpesvirus 6 (HHV-6) was found to up-regulate expression of human immunodeficiency virus and human T cell leukaemia virus type I (HTLV-I) long terminal repeat sequence (LTR), and herpes simplex virus type 1 (HSV-1) gD chloramphenicol acetyltransferase (CAT) constructs transfected into the T cell line, J. Jhan. Activation by HHV-6 was due to one or more viral proteins produced early in infection and, in the case of the HTLV-I LTR, was synergistic to induction mediated by the HTLV-I tax gene product. Neither the HTLV-I enhancer nor basal promoter elements of the HSV-1 gD gene were essential for activation and no increase in accumulated HTLV-I mRNA was observed due to HHV-6 infection. Induction by HHV-6 was found to be dependent on the reporter construct used, because the CAT gene and, to a lesser extent, the HSV-1 thymidine kinase gene were responsive to HHV-6 infection although no significant activation of growth hormone constructs was observed. Our results bear a strong resemblance to those obtained for the Epstein-Barr virus BMLF1 gene, indicating that the major HHV-6 trans-activator may be a homologue of this gene.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Everett, Professor Roger and Onions, Professor David
Authors: Campbell, M.E.M., McCorkindale, S., Everett, R.D., and Onions, D.E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of General Virology
Journal Abbr.:J. Gen. Virol.
Publisher:Society for General Microbiology
ISSN (Online):1465-2099

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