Mutational dissection of the 21 bp repeat region of the SV40 early promoter reveals that it contains overlapping elements of the early-early and late-early promoters

Baty, D., Barrera-Saldana, H.A., Everett, R.D., Vigneron, M. and Chambon, P. (1984) Mutational dissection of the 21 bp repeat region of the SV40 early promoter reveals that it contains overlapping elements of the early-early and late-early promoters. Nucleic Acids Research, 12(2), pp. 915-932. (doi: 10.1093/nar/12.2.915)

Abstract

Using quantitative S1 nuclease analysis and recombinants which contain the SV4O early promoter region linked to the rabbit β -globin gene coding sequence, we have studied the effect of deletion, inversion and point mutations, located within the 21 bp repeat region, on initiation of transcription from the early-early and late-early startsites in the absence of T-antigen. Our data establish unequivocally that the six GC-rich repeats present in the 21 bp repeat region are essential elements of both the early-early and late-early promoters and that they are not redundant, since mutations, which affect only the GC-rich repeat most proximal to the TATA box, decrease drastically the activity of the early-early promoter, but increase that of the late-early promoter. On the other hand, the four GC-rich repeats most proximal to the 72 bp repeat are common elements of the two overlapping early-early and late-early promoters. Our results, which confirm that the early-early promoter is stronger than the late-early one, also support our previous suggestion that they are in competition for the transcriptional machinery. The general organization of the SV4O early promoter region is discussed.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Everett, Professor Roger
• Authors: Baty, D., Barrera-Saldana, H.A., Everett, R.D., Vigneron, M., and Chambon, P.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Nucleic Acids Research
• Journal Abbr.: Nucl. Acids Res.
• ISSN: 0305-1048
• ISSN (Online): 1362-4962