SUMO pathway dependent recruitment of cellular repressors to herpes simplex virus type 1 genomes

Cuchet-Lourenço, D., Boutell, C. , Lukashchuk, V., Grant, K., Sykes, A. , Murray, J., Orr, A. and Everett, R.D. (2011) SUMO pathway dependent recruitment of cellular repressors to herpes simplex virus type 1 genomes. PLoS Pathogens, 7(7), e1002123. (doi: 10.1371/journal.ppat.1002123) (PMID:21779164) (PMCID:PMC3136452)

[img]
Preview
Text
72783.pdf
Available under License Creative Commons Attribution.

1MB

Abstract

Components of promyelocytic leukaemia (PML) nuclear bodies (ND10) are recruited to sites associated with herpes simplex virus type 1 (HSV-1) genomes soon after they enter the nucleus. This cellular response is linked to intrinsic antiviral resistance and is counteracted by viral regulatory protein ICP0. We report that the SUMO interaction motifs of PML, Sp100 and hDaxx are required for recruitment of these repressive proteins to HSV-1 induced foci, which also contain SUMO conjugates and PIAS2β, a SUMO E3 ligase. SUMO modification of PML and elements of its tripartite motif (TRIM) are also required for recruitment in cells lacking endogenous PML. Mutants of PML isoform I and hDaxx that are not recruited to virus induced foci are unable to reproduce the repression of ICP0 null mutant HSV-1 infection mediated by their wild type counterparts. We conclude that recruitment of ND10 components to sites associated with HSV-1 genomes reflects a cellular defence against invading pathogen DNA that is regulated through the SUMO modification pathway.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Grant, Dr Kyle and Everett, Professor Roger and Orr, Mrs Anne and Sykes, Dr Amanda and Boutell, Dr Chris and Cuchet, Dr Delphine and Murray, Mrs Jill
Authors: Cuchet-Lourenço, D., Boutell, C., Lukashchuk, V., Grant, K., Sykes, A., Murray, J., Orr, A., and Everett, R.D.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
University Services > Learning and Teaching Services Division
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation > Centre for Virus Research
Journal Name:PLoS Pathogens
Publisher:Public Library of Science
ISSN:1553-7366
ISSN (Online):1553-7374
Copyright Holders:Copyright © 2011 The Authors
First Published:First published in PLoS ONE 7(7):e100212
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

University Staff: Request a correction | Enlighten Editors: Update this record