Intramembrane proteolysis promotes trafficking of hepatitis C virus core protein to lipid droplets

McLauchlan, J., Lemberg, M.K., Hope, G. and Martoglio, B. (2002) Intramembrane proteolysis promotes trafficking of hepatitis C virus core protein to lipid droplets. EMBO Journal, 21(15), pp. 3980-3988. (doi:10.1093/emboj/cdf414)

Full text not currently available from Enlighten.

Abstract

Hepatitis C virus (HCV) is the major causative pathogen associated with liver cirrhosis and hepatocellular carcinoma. The virus has a positive-sense RNA genome encoding a single polyprotein with the virion components located in the N-terminal portion. During biosynthesis of the polyprotein, an internal signal sequence between the core protein and the envelope protein E1 targets the nascent polypeptide to the endoplasmic reticulum (ER) membrane for translocation of E1 into the ER. Following membrane insertion, the signal sequence is cleaved from E1 by signal peptidase. Here we provide evidence that after cleavage by signal peptidase, the signal peptide is further processed by the intramembrane-cleaving protease SPP that promotes the release of core protein from the ER membrane. Core protein is then free for subsequent trafficking to lipid droplets. This study represents an example of a potential role for intramembrane proteolysis in the maturation of a viral protein.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hope, Dr Graham and McLauchlan, Professor John
Authors: McLauchlan, J., Lemberg, M.K., Hope, G., and Martoglio, B.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:EMBO Journal
ISSN:0261-4189
ISSN (Online):1460-2075

University Staff: Request a correction | Enlighten Editors: Update this record