Intraocular T cells of patients with herpes simplex virus (HSV)–induced acute retinal necrosis recognize HSV tegument proteins VP11/12 and VP13/14

Verjans, G.M.G.M., Dings, M.E.M., McLauchlan, J. , van der Kooi, A., Hoogerhout, P., Brugghe, H.F., Timmermans, H.A.M., Baarsma, G.S. and Osterhaus, A.D.M.E. (2000) Intraocular T cells of patients with herpes simplex virus (HSV)–induced acute retinal necrosis recognize HSV tegument proteins VP11/12 and VP13/14. Journal of Infectious Diseases, 182(3), pp. 923-927. (doi: 10.1086/315759)

Abstract

It has previously been shown that T cells specific for the triggering virus infiltrate the eye of patients with herpes simplex virus type 1 (HSV-1)—induced acute retinal necrosis (ARN). The T cells were mainly directed against 0.67–0.73 HSV-1 map region encoded antigens. The fine specificities of genetically different T cell clones (TCC), obtained from affected eyes of 3 patients with HSV-induced ARN and reactive toward this genomic region of HSV-1, were analyzed with recombinant HSV viruses and synthetic peptides. For 1 patient, the HSV-1 UL46 gene encoded tegument protein VP11/12 was identified as the target antigen. Two separate CD4⁺ T cell epitopes were denned in VP11/12. TCC from the other 2 patients recognized the HSV-1 UL47 gene encoded tegument protein VP13/14. Two separate CD4⁺ VP13/14 T cell epitopes were identified in these patients. Analysis of the data indicates that HSV-1 VP11/12 and VP13/14 are major target antigens for T cells obtained from vitreous fluid samples of the HSV-induced ARN patients studied.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: McLauchlan, Professor John
• Authors: Verjans, G.M.G.M., Dings, M.E.M., McLauchlan, J., van der Kooi, A., Hoogerhout, P., Brugghe, H.F., Timmermans, H.A.M., Baarsma, G.S., and Osterhaus, A.D.M.E.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
• Journal Name: Journal of Infectious Diseases
• Journal Abbr.: J Infect Dis.
• ISSN: 0022-1899
• ISSN (Online): 1537-6613