Enlighten
Research publications by members of the University of Glasgow
home > services > Enlighten

Sequence motifs required for lipid droplet association and protein stability are unique to the hepatitis C virus core protein

Hope, R.G., and McLauchlan, J. (2000) Sequence motifs required for lipid droplet association and protein stability are unique to the hepatitis C virus core protein. Journal of General Virology, 81 (Pt 8). pp. 1913-25. ISSN 0022-1317

Full text not currently available from Enlighten.

Publisher's URL: http://vir.sgmjournals.org/content/81/8/1913.abstract

Abstract

From analysis of the primary sequence of the hepatitis C virus (HCV) core protein, we have identified three separable regions based on hydrophobicity and clustering of basic amino acids within the protein. Comparison with capsid proteins of related pesti- and flaviviruses suggested that HCV core has a unique central domain (domain 2). Previous findings have revealed that core protein can associate with lipid droplets which are intracellular storage sites for triacylglycerols and cholesterol esters. Confocal analysis of variant forms lacking regions of core indicated that most residues within the unique region are necessary for association of the protein with lipid droplets. A segment within domain 2 (from residues 125 to 144) also was required for stability of the protein and a polypeptide lacking these sequences was degraded apparently by the proteasome. In cells depleted of lipid droplets, core protein remained located in the cytoplasm. Moreover, cleavage of the protein at the maturation site and stability were not affected by inability to bind to lipid droplets.

Item Type:Article
Status:Published
Refereed:Yes
Glasgow Author(s):McLauchlan, Dr John
Authors: Hope, R.G., and McLauchlan, J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of General Virology
Journal Abbr.:J. Gen. Virol.
Publisher:Society for General Microbiology
ISSN:0022-1317
ISSN (Online):1465-2099

University Staff: Request a correction | Enlighten Editors: Update this record