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Publisher's URL: http://dx.doi.org/10.1099/0022-1317-73-3-521
The protein predicted to be encoded by varicella-zoster virus (VZV) gene 61 exhibits limited amino acid sequence similarity to the herpes simplex virus type 1 nuclear phosphoprotein Vmw 110, which functions as a transcriptional activator. The gene 61 protein was expressed in its entirety, or as an amino- or carboxyterminal fragment in Escherichia coli and vaccinia virus recombinants, and monospecific rabbit antisera were raised against an E. coli fusion between fl-galactosidase and the majority of the gene 61 protein. Use of the antisera showed that the gene 61 protein is present in VZV-infected cell nuclei as a heterogeneous phosphoprotein of Mr 62K to 65K. Phosphorylation occurs in the amino- and, to a lesser extent, carboxy-terminal portions of the protein. The carboxy-terminal region directs transport of the protein to the nucleus, whereas the amino-terminal region, which contains a potential zinc-binding domain, is responsible for a punctate distribution. Preliminary mapping data indicated that gene 61 is transcribed as a 1.8 kb mRNA which initiates about 65 bp upstream from the translation initiation codon, at a position located appropriately with respect to potential regulatory elements.
|Glasgow Author(s) Enlighten ID:||Davison, Dr Andrew|
|Authors:||Stevenson, D., Colman, K.L., and Davison, A.J.|
|College/School:||College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation|
|Journal Name:||Journal of General Virology|
|Journal Abbr.:||J. Gen. Virol.|
|Publisher:||Society for General Microbiology|
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