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Recombinants between herpes simplex virus types 1 and 2: analyses of genome structures and expression of immediate early polypeptides

Preston, V.G., Davison, A.J., Marsden, H.S., Timbury, M.C., Subak-Sharpe, J.H., and Wilkie, N.M. (1978) Recombinants between herpes simplex virus types 1 and 2: analyses of genome structures and expression of immediate early polypeptides. Journal of Virology, 28 (2). pp. 499-517. ISSN 0022-538X

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Publisher's URL: http://jvi.asm.org/content/28/2/499.abstract

Abstract

Recombinants between temperature-sensitive mutants of herpes simplex virus types 1 (HSV-1) and 2 (HSV-2) were constructed. Using restriction endonucleases, we analyzed the genome composition of 17 intertypic recombinants and detected crossovers in every region of the genome. The virion DNA of one recombinant appeared to be largely "frozen" in two of the four possible genome arrangements of HSV. Knowledge of the genome structures of recombinants enabled us to physically map immediate early polypeptides. We present evidence that the immediate early polypeptide Vmw IE 110 of HSV-1 and its functionally equivalent polypeptide, Vmw IE 118, of HSV-2 may map in the repetitive sequences bounding the long unique region of HSV.

Item Type:Article
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Davison, Dr Andrew and Preston, Dr Valerie
Authors: Preston, V.G., Davison, A.J., Marsden, H.S., Timbury, M.C., Subak-Sharpe, J.H., and Wilkie, N.M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Virology
Journal Abbr.:J. Virol.
ISSN:0022-538X
ISSN (Online):1098-5514

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