Preston, V.G., Davison, A.J., Marsden, H.S., Timbury, M.C., Subak-Sharpe, J.H., and Wilkie, N.M. (1978) Recombinants between herpes simplex virus types 1 and 2: analyses of genome structures and expression of immediate early polypeptides. Journal of Virology, 28 (2). pp. 499-517. ISSN 0022-538X
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Publisher's URL: http://jvi.asm.org/content/28/2/499.abstract
Recombinants between temperature-sensitive mutants of herpes simplex virus types 1 (HSV-1) and 2 (HSV-2) were constructed. Using restriction endonucleases, we analyzed the genome composition of 17 intertypic recombinants and detected crossovers in every region of the genome. The virion DNA of one recombinant appeared to be largely "frozen" in two of the four possible genome arrangements of HSV. Knowledge of the genome structures of recombinants enabled us to physically map immediate early polypeptides. We present evidence that the immediate early polypeptide Vmw IE 110 of HSV-1 and its functionally equivalent polypeptide, Vmw IE 118, of HSV-2 may map in the repetitive sequences bounding the long unique region of HSV.
|Glasgow Author(s):||Davison, Dr Andrew and Preston, Dr Valerie|
|Authors:||Preston, V.G., Davison, A.J., Marsden, H.S., Timbury, M.C., Subak-Sharpe, J.H., and Wilkie, N.M.|
|College/School:||College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation|
|Journal Name:||Journal of Virology|
|Journal Abbr.:||J. Virol.|