Experience in shotgun sequencing a 134 kilobase pair DNA molecule

Davison, A.J. (1991) Experience in shotgun sequencing a 134 kilobase pair DNA molecule. DNA Sequence: The Journal of DNA Sequencing and Mapping, 1(6), pp. 389-394.

Full text not currently available from Enlighten.

Abstract

Until now, large DNA sequences have been obtained by cloning fragments of the target molecule into plasmid, cosmid or bacteriophage lambda vectors. The 134 kbp DNA sequence of channel catfish virus was determined with relative ease by shotgun cloning of random fragments of genomic DNA directly into a bacteriophage M13 vector, sequencing by dideoxynucleotide chain termination, and compilation of the data using Staden's database handling programs. Experience gained during this endeavour indicates that sequences substantially larger than 134 kbp may be obtained using this approach.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Davison, Professor Andrew
Authors: Davison, A.J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:DNA Sequence: The Journal of DNA Sequencing and Mapping
ISSN:1042-5179

University Staff: Request a correction | Enlighten Editors: Update this record