Davison, A.J., and Wilkie, N.M. (1983) Inversion of the two segments of the Herpes Simplex virus genome in intertypic recombinants. Journal of General Virology, 64 (1). pp. 1-18. ISSN 0022-1317 (doi:10.1099/0022-1317-64-1-1)
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Publisher's URL: http://dx.doi.org/10.1099/0022-1317-64-1-1
We have analysed by restriction site mapping the structures of the termini and L-S joint in several HSV-1/HSV-2 intertypic recombinants, including Bx1(28-1), the virion DNA of which has a marked overabundance of one orientation of the L segment, and subclones of Bx1(28-1). All recombinants with both orientations of L present in equal amounts contain TRL and IRL regions derived at least in part from the same parent (HSV-1 or HSV-2) as a result of previously undetected crossovers in these regions. Recombinants with a predominance of one orientation of L have TRL and IRL regions derived from different parents. Homology between a sequences alone at the L terminus and L-S joint is sufficient for normal inversion of L. Analysis of another recombinant, RE4, which fails to invert normally in both L and S, suggests that normal inversion of S is dependent upon the presence of TRS and IRS regions derived at least in part from the same parent. We conclude that segment inversion specifically depends upon the a sequence, that the process of DNA replication and maturation does not necessarily produce molecules with identical a sequences, and that direct ligation of termini may occur during DNA replication.
|Glasgow Author(s):||Davison, Dr Andrew|
|Authors:||Davison, A.J., and Wilkie, N.M.|
|College/School:||College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation|
|Journal Name:||Journal of General Virology|
|Journal Abbr.:||J. Gen. Virol.|
|Publisher:||Society for General Microbiology|