Genomic studies of the Lucke tumor herpesvirus (RaHV-1)

Davison, A.J. , Sauerbier, W., Dolan, A., Addison, C. and McKinnell, R.G. (1999) Genomic studies of the Lucke tumor herpesvirus (RaHV-1). Journal of Cancer Research and Clinical Oncology, 125(3-4), pp. 232-238. (doi: 10.1007/s004320050268)

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Ranid herpesvirus 1 (RaHV-1) is the etiological agent of the Lucké renal adenocarcinoma of the North American leopard frog Rana pipiens. Construction of cosmid libraries containing RaHV-1 DNA inserts allowed the derivation of a BamHI map for the viral genome. Summation of fragment sizes indicates that the genome is 217 kbp in size, a value in accordance with the most recent published estimate (220 kbp) obtained by field-inversion gel electrophoresis. The DNA sequence of the 39,757-bp insert in 1 cosmid (cos54) was determined and was predicted to contain 21 complete and 3 partial genes. In all, 12 genes have distant counterparts in a fish herpesvirus (ictalurid herpesvirus 1) and are present in 2 blocks, 1 of which is relatively inverted. This indicates that RaHV-1 belongs to the fish virus lineage of the herpesvirus family rather than to the lineage populated by mammalian and avian viruses. The remainding 12 genes in cos54 lack counterparts in any other herpesvirus. One of these encodes a putative DNA (cytosine-5) methyltransferase. This raises the possibility that biological processes induced in the host by RaHV-1 might involve methylation of cellular DNA by the viral enzyme.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Addison, Mrs Clare and Davison, Professor Andrew and Dolan, Mr Aidan
Authors: Davison, A.J., Sauerbier, W., Dolan, A., Addison, C., and McKinnell, R.G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Cancer Research and Clinical Oncology
ISSN (Online):1432-1335

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