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The DNA sequence of the equine herpesvirus 4 gene encoding glycoprotein gp17/18, the homologue of herpes simplex virus glycoprotein gD

Cullinane, A.A., Neilan, J., Wilson, L., Davison, A.J., and Allen, G. (1993) The DNA sequence of the equine herpesvirus 4 gene encoding glycoprotein gp17/18, the homologue of herpes simplex virus glycoprotein gD. Journal of General Virology, 74 (9). pp. 1959-1964. ISSN 0022-1317 (doi:10.1099/0022-1317-74-9-1959)

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Publisher's URL: http://dx.doi.org/10.1099/0022-1317-74-9-1959

Abstract

The nucleotide sequence of the gene to the left of the gI gene of equine herpesvirus 4 (EHV-4) was determined. The gene encodes a peptide of 402 amino acids with an unprocessed M r of 45 323. The predicted polypeptide has several features of a glycoprotein including a hydrophobic signal sequence, a membrane spanning domain and four potential N-linked glycosylation sites within the proposed external domain. The predicted amino acid sequence of EHV-4 gD shows 83 % identity with that of equine herpesvirus 1 gD. Conservation of the tertiary structure is suggested by the alignment of six cysteine residues with those of the gD of six other alphaherpesviruses. Screening a 2gtl 1/EHV-4 expression library with monoclonal antibodies against several of the most abundant EHV-4 glycoproteins unequivocally identified the protein encoded by the EHV-4 gD gene as gp17/18.

Item Type:Article
Status:Published
Refereed:Yes
Glasgow Author(s):Davison, Dr Andrew
Authors: Cullinane, A.A., Neilan, J., Wilson, L., Davison, A.J., and Allen, G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of General Virology
Journal Abbr.:J. Gen. Virol.
Publisher:Society for General Microbiology
ISSN:0022-1317
ISSN (Online):1465-2099

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