Cullinane, A.A., Neilan, J., Wilson, L., Davison, A.J., and Allen, G. (1993) The DNA sequence of the equine herpesvirus 4 gene encoding glycoprotein gp17/18, the homologue of herpes simplex virus glycoprotein gD. Journal of General Virology, 74 (9). pp. 1959-1964. ISSN 0022-1317 (doi:10.1099/0022-1317-74-9-1959)
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Publisher's URL: http://dx.doi.org/10.1099/0022-1317-74-9-1959
The nucleotide sequence of the gene to the left of the gI gene of equine herpesvirus 4 (EHV-4) was determined. The gene encodes a peptide of 402 amino acids with an unprocessed M r of 45 323. The predicted polypeptide has several features of a glycoprotein including a hydrophobic signal sequence, a membrane spanning domain and four potential N-linked glycosylation sites within the proposed external domain. The predicted amino acid sequence of EHV-4 gD shows 83 % identity with that of equine herpesvirus 1 gD. Conservation of the tertiary structure is suggested by the alignment of six cysteine residues with those of the gD of six other alphaherpesviruses. Screening a 2gtl 1/EHV-4 expression library with monoclonal antibodies against several of the most abundant EHV-4 glycoproteins unequivocally identified the protein encoded by the EHV-4 gD gene as gp17/18.
|Glasgow Author(s):||Davison, Dr Andrew|
|Authors:||Cullinane, A.A., Neilan, J., Wilson, L., Davison, A.J., and Allen, G.|
|College/School:||College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation|
|Journal Name:||Journal of General Virology|
|Journal Abbr.:||J. Gen. Virol.|
|Publisher:||Society for General Microbiology|