Bradley, A.J., Lurain, N.S., Ghazal, P., Trivedi, U., Cunningham, C., Baluchova, K., Gatherer, D., Wilkinson, G.W.G., Dargan, D.J. and Davison, A.J. (2009) High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169. Journal of General Virology, 90(10), pp. 2375-2380. (doi: 10.1099/vir.0.013250-0) (PMID:19553388) (PMCID:PMC2885757)
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Abstract
The genomes of commonly used variants of human cytomegalovirus (HCMV) strains Towne and AD169 each contain a substantial mutation in which a region (UL/b′) at the right end of the long unique region has been replaced by an inverted duplication of a region from the left end of the genome. Using high-throughput technology, we have sequenced HCMV strain Towne (ATCC VR-977) and confirmed the presence of two variants, one exhibiting the replacement in UL/b′ and the other intact in this region. Both variants are mutated in genes RL13, UL1, UL40, UL130, US1 and US9. We have also sequenced a novel AD169 variant (varUC) that is intact in UL/b′ except for a small deletion that affects genes UL144, UL142, UL141 and UL140. Like other AD169 variants, varUC is mutated in genes RL5A, RL13, UL36 and UL131A. A subpopulation of varUC contains an additional deletion affecting genes IRS1, US1 and US2.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Gatherer, Dr Derek and Davison, Professor Andrew and Cunningham, Mr Charles and Dargan, Dr Derrick and Baluchova, Dr Katarina |
Authors: | Bradley, A.J., Lurain, N.S., Ghazal, P., Trivedi, U., Cunningham, C., Baluchova, K., Gatherer, D., Wilkinson, G.W.G., Dargan, D.J., and Davison, A.J. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Journal of General Virology |
Journal Abbr.: | J. Gen. Virol. |
Publisher: | Society for General Microbiology |
ISSN: | 0022-1317 |
ISSN (Online): | 1465-2099 |
Published Online: | 24 June 2009 |
Copyright Holders: | Copyright © 2009 SGM |
First Published: | First Published in Journal of General Virology 90(10):2375-2380 |
Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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