High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169

Bradley, A.J., Lurain, N.S., Ghazal, P., Trivedi, U., Cunningham, C., Baluchova, K., Gatherer, D., Wilkinson, G.W.G., Dargan, D.J. and Davison, A.J. (2009) High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169. Journal of General Virology, 90(10), pp. 2375-2380. (doi:10.1099/vir.0.013250-0) (PMID:19553388) (PMCID:PMC2885757)

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Abstract

The genomes of commonly used variants of human cytomegalovirus (HCMV) strains Towne and AD169 each contain a substantial mutation in which a region (UL/b′) at the right end of the long unique region has been replaced by an inverted duplication of a region from the left end of the genome. Using high-throughput technology, we have sequenced HCMV strain Towne (ATCC VR-977) and confirmed the presence of two variants, one exhibiting the replacement in UL/b′ and the other intact in this region. Both variants are mutated in genes RL13, UL1, UL40, UL130, US1 and US9. We have also sequenced a novel AD169 variant (varUC) that is intact in UL/b′ except for a small deletion that affects genes UL144, UL142, UL141 and UL140. Like other AD169 variants, varUC is mutated in genes RL5A, RL13, UL36 and UL131A. A subpopulation of varUC contains an additional deletion affecting genes IRS1, US1 and US2.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Baluchova, Dr Katarina and Cunningham, Mr Charles and Dargan, Dr Derrick and Gatherer, Dr Derek and Davison, Professor Andrew
Authors: Bradley, A.J., Lurain, N.S., Ghazal, P., Trivedi, U., Cunningham, C., Baluchova, K., Gatherer, D., Wilkinson, G.W.G., Dargan, D.J., and Davison, A.J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of General Virology
Journal Abbr.:J. Gen. Virol.
Publisher:Society for General Microbiology
ISSN:0022-1317
ISSN (Online):1465-2099
Published Online:24 June 2009
Copyright Holders:Copyright © 2009 SGM
First Published:First Published in Journal of General Virology 90(10):2375-2380
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
515351Establishment of an MRC Sequencing Hub at the GenePool, the Scottish next-generation genomics facilityPawel HerzykMedical Research Council (MRC)G0900740/91082RI MOLECULAR CELL & SYSTEMS BIOLOGY
656321Genomics of human cytomegalovirusAndrew DavisonMedical Research Council (MRC)MC_UU_12014/3MVLS III - CENTRE FOR VIRUS RESEARCH